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Juvenile Tissue Citrus Transformation Facility

Juvenile Tissue Citrus Transformation Facility


The Juvenile Tissue Citrus Transformation Facility (JTCTF) has undergone a transition to Educational Business Activity unit in accordance with request made by UF Administration. This transition completely overhauls the operation of the facility and brings into force new set of rules in regard to ordering, pricing, and payments to the JTCTF for the services it provides.

Mission
The Juvenile Tissue Citrus Transformation Facility was established to expedite integration of recombinant DNA technologies and genetic engineering into basic research from other related disciplines, including plant pathology, biochemistry, food science/processing, post-harvest physiology, horticulture, and entomology by providing a service that allows researchers to have their genes of interest transferred and tested in the appropriate citrus cultivars. A functional core citrus transformation laboratory benefits nearly all phases of the Florida citrus industry and plays a major role in the long-term improvement of citrus.

Purpose
The purpose of the JTCTF is to produce genetically modified Citrus plants. The facility is equipped to employ two methods of genetic transformation: Agrobacterium-mediated transformation and biolistic particle bombardment. At this time we are producing transgenic plants only through Agrobacterium-mediated transformation. This method utilizes naturally occurring pathogenic bacterium called Agrobacterium tumefaciens that has the ability to insert the fragment of DNA into the genome of host plant. These bacteria have been manipulated and rendered non-pathogenic although they kept their ability to transfer foreign DNA into host’s genome. The DNA fragment of interest to our client is introduced into Agrobacterium, and such newly created strain is used for treatment of plant tissues that will result in production of plantlets that have their genomes altered by the presence of foreign DNA. Additional tests are performed to confirm that genetic modification took place.