Transformation Services

Mature Citrus Facility

Options & Prices for Transgenic Plants

We produce early flowering, transgenic and cis/intragenic mature citrus for research, field testing, and potential commercialization.

Ordering transgenic plants from the Mature Citrus Facility (MCF)

i). DNA of the vector is preferentially accepted and the MCF can transform it into Agrobacterium.

For commercialization of transgenics, the gus reporter gene, not gfp, should be used with nptII selection. The pCAMBIA2301 vector is recommended and can be provided prior to sub-cloning. At this time, the gfp reporter gene has not been deregulated for commercialization. If a transgenic plant with gfp looks promising in research, the transgenic must be remade with gus for commercialization.  However, the gfp reporter gene and nptII selectable marker can be used for research purposes. 

ii). We can sub-clone gene fragments into pCAMBIA2301 (discuss price with us).

Options & Prices:

Transformation Method*

Line-Item Service

 

 

Price UF
    Internal*

      Price External Academic*

      Price External Market*

Agrobacterium (easily transformed Hamlin, Early Valencia 1 (EV1), Kuharske cultivars)

Seeding & transplanting/ batch (3 months of growth)

   2x 97.90

   285.20

   313.70

 

 

Budding/batch (3 months of growth)

   2x 147.00

   365.60

   402.20

 

 

Transformation/batch

   243.70

   647.40

   712.10

 

 

Micrografting/1 shoot

   20.90

   35.50

   39.00

 

 

 

Secondary grafting/1 shoot

   16.50

   30.90

 

   34.00

 

 

Price for plant w/ Agro*

   770.90

2,015.00

2,216.90

 

 

 

 

 

 

 

 

 

 

Additional services

PCR/batch

   207.70

  476.50

   524.20

 

 

GUS assay/batch

   104.80

  234.20

 

   257.60

 

Difficult cultivar charge

e.g., OLL8, Vernia, some grapefruits

   396.90

1,619.00

 

1,780.90

 

Custom service (discuss with us)

qPCR, mutation detection, propagation

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*Note: The transformation process involves seeding, transplanting, budding, transformation & screening, micrografting, & secondary grafting line-item services. Transformations of scions require 2x batches of seeding & 2x batches of budding, while rootstock with an overexpression vector (not CRISPR) usually requires 1x batch.

*Note: If all transgenics can be obtained from one transformation, charges only accrue for the transformation once. Note: GUS is for commercialization, whereas GFP is for research.