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Core Citrus Transformation Facility

Citrus Transformation Phases

Sterilized seeds are allowed to germinate in darkness for 4-5 weeks

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Sterilized seeds are allowed to germinate
in darkness for 4-5 weeks.



Stems of seedlings

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Stems of seedlings grown in vitro are
cut into 1/2 inch explants, incubated with
Agrobacteria and placed on shoot
regeneration medium

Shoots appear in explants (4-5 weeks after treatment with bacteria)

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Shoots appear in explants (4-5 weeks after treatment with bacteria)

Shoots are collected from explants

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Shoots are collected from explants
and allowed to grow for a week

Tested for reporter genes

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Tested for reporter genes

Shoots grafted on rootstocks in vitro

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Shoots grafted on rootstocks in vitro

Two weeks old, soil adapted seedlings

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Two weeks old, soil adapted seedlings

Four months seedlings

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Four months seedlings

Gel with the products of PCR reaction proving that the gene of interest is present within the tissue of transgenic plants

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Gel with the products of PCR reaction proving that the gene of interest is present within the tissue of transgenic plants