Skip to main content

UF/IFAS Citrus Research and Education Center

UF/IFAS Citrus Research and Education Center

Publications - Dr. Manjul Dutt

Refereed Publications

Mahmoud L.M., P. Kaur, D. Stanton, J.W. Grosser and M. Dutt 2022. A cationic lipid mediated CRISPR/Cas9 technique for the production of stable genome edited citrus plants. Plant methods 18 (1):1-14.

Welker S., M. Pierre, J.P. Santiago, M. Dutt, C. Vincent and A. Levy. 2022. Phloem transport limitation in Huanglongbing-affected sweet orange is dependent on phloem-limited bacteria and callose. Tree Physiology 42 (2):379-390.

Canton M., H. Wu, M. Dutt and J.Zale. 2022. A new liquid selection system for mature citrus transformation. Scientia Horticulturae 293: 110672.

Mahmoud L.M., D. Stanton, B.H. Amin, J.W. Grosser and M. Dutt. 2022. Overexpression of the Arabidopsis NPR1 gene confers enhanced salt tolerance by regulating antioxidant and starch accumulation in citrus. Plant Cell, Tissue and Organ Culture (PCTOC):1-13.

Wu H., M. Canton, L.M. Mahmoud, K.R. Weber, G.Z. Michalczyk, M. Dutt, J.M. Zale 2022. Identification and Characterization of Two Putative Citrus Phosphomannose Isomerase (CsPMI) Genes as Selectable Markers for Mature Citrus Transformation. Horticulturae 8 (3):204. 

Dutt M., L.M. Mahmoud, K. Chamusco, D. Stanton, C.D. Chase, E. Nielsen, M. Quirico, Q. Yu, F.G. Gmitter Jr and J.W. Grosser. 2021. Utilization of somatic fusion techniques for the development of HLB tolerant breeding resources employing the Australian finger lime (Citrus australasica). Plos one 16 (8):e0255842. 

Mahmoud L.M., A.M. Shalan, M.S. El-Boray, C.I. Vincent, M.E. El-Kady, J.W. Grosser and M. Dutt. 2022. Application of silicon nanoparticles enhances oxidative stress tolerance in salt stressed ‘Valencia’ sweet orange plants. Scientia Horticulturae 295:110856.

Adhikari B., M. Dutt, T. Vashisth. 2021. Comparative phytochemical analysis of the fruits of four Florida-grown finger lime (Citrus australasica) selections. LWT 135:110003.

Mahmoud L.M., P.J. Huyck, C.I. Vincent, F.G. Gmitter Jr, J.W. Grosser, and M. Dutt. 2021. Physiological Responses and Gene Expression Patterns in Open-Pollinated Seedlings of a Pummelo-Mandarin Hybrid Rootstock Exposed to Salt Stress and Huanglongbing. Plants 10 (7):1439. 

Mahmoud L.M., C.I. Vincent, J.W. Grosser, and M. Dutt. 2021. The response of salt-stressed Valencia sweet orange (Citrus sinensis) to salicylic acid and methyl jasmonate treatments. Plant Physiology Reports 26 (1):137-151.

Dutt M., Z. Mou, X. Zhang, S.E. Tanwir and J.W. Grosser. 2020. Efficient CRISPR/Cas9 genome editing with Citrus embryogenic cell cultures. BMC biotechnology 20 (1):1-7.

Soares J.M., K.C. Weber, W. Qiu, D. Stanton, L.M. Mahmoud, H. Wu, P. Huyck, J. Zale, K. Al Jasim, J.W. Grosser and M. Dutt. 2020. The vascular targeted citrus FLOWERING LOCUS T3 gene promotes non-inductive early flowering in transgenic Carrizo rootstocks and grafted juvenile scions. Scientific reports 10 (1):1-18.

Soares J.M., S. E. Tanwir, J.W. Grosser and M. Dutt. 2020. Development of genetically modified citrus plants for the control of citrus canker and huanglongbing. Tropical Plant Pathology 45, 237–250.

Mahmoud L.M., M. Dutt, C.I. Vincent and J.W. Grosser. 2020. Salinity-induced physiological responses of three putative salt tolerant citrus rootstocks. Horticulturae 6 (4):90. 

Mahmoud L.M., J.W. Grosser and M. Dutt. 2020. Silver compounds regulate leaf drop and improve in vitro regeneration from mature tissues of Australian finger lime (Citrus australasica). Plant Cell, Tissue and Organ Culture (PCTOC) 141 (3):455-464.

Corte, L.E., B.M.J. Mendes, F.A.A. Mourao Filho, J.W. Grosser and M. Dutt (2020). Functional characterization of full-length and 5′ deletion fragments of Citrus sinensis-derived constitutive promoters in Nicotiana benthamiana. In Vitro Cellular and Developmental Biology – Plant

Qiu, W., J. Soares, Z. Pang, Y. Huang, Z. Sun, N. Wang, J.W. Grosser and M. Dutt (2020). Potential Mechanisms of AtNPR1 Mediated Resistance against Huanglongbing (HLB) in Citrus. International journal of molecular sciences 2020212009.

Achor, D., S. Welker, S. Ben-Mahmoud, C. Wang, S. Y. Folimonova, M. Dutt, S. Gowda, and A. Levy (2020). Dynamics of Candidatus Liberibacter asiaticus movement and sieve-pores plugging in citrus sink cells. Plant Physiology 182(2):882-891

Erpen-Dalla Corte, L., L. M. Mahmoud, T. S. Moraes, Z. Mou, J. W. Grosser and M. Dutt (2019) Development of improved fruit, vegetable and ornamental crops using the CRISPR/Cas9 genome editing technique. Plants, 8, 601

Liu, Z., X. X.  Ge, W. Qiu, J. M. Long, H. H. Jia, W. Yang, M. Dutt, X. M. Wu and W Guo. 2018. Overexpression of a B3 transcription factor CsFUS3 promotes somatic embryogenesis in CitrusPlant Science 277: 121-131.

Dutt, M., L. Erpen, and J.W. Grosser, 2018. Genetic transformation of the ‘W Murcott’ tangor: comparison between different techniques. Scientia Horticulturae, 242:90-94.

Hijaz, F., Y., Nehela, S. E., Jones, M. Dutt, J. W., Grosser, J. A., Manthey and N. Killiny. 2018. Metabolically engineered anthocyanin-producing lime provides additional nutritional value and antioxidant potential to juice. Plant Biotechnology Reports, 12(5):329-346.

Kaur, P., P. Gonzalez, M. Dutt, and E. Etxeberria. 2018. Identification of sieve elements and companion cell protoplasts by a combination of brightfield and fluorescence microscopy. Applications in Plant Sciences 6(9): e1179.

Erpen, L., E.C.R. Tavano, R. Harakava, M. Dutt, J.W. Grosser, S.M.S. Piedade, B.M.J. Mendes and F.A.A. Mourao Filho (2018) Isolation, characterization, and evaluation of three Citrus sinensis-derived constitutive gene promoters. Plant Cell Reports 37(8): 1113–1125.

Killiny N., S.E. Jones, Y. Nehela, F. Hijaz, M. Dutt, F.G. Gmitter and J.W. Grosser (2018) All roads lead to Rome: Towards understanding different avenues of tolerance to huanglongbing in citrus cultivars. Plant Physiology and Biochemistry129:1-10.

Dutt M., F.T. Zambon, L. Erpen, L. Soriano, J.W. Grosser (2018). Embryo-specific expression of a visual reporter gene as a selection system for citrus transformation. PLOS ONE 13 (1):e0190413..pone.0190413.

Erpen L., H.S. Devi, J.W. Grosser and M. Dutt (2018). Potential use of the DREB/ERF, MYB, NAC and WRKY transcription factors to improve abiotic and biotic stress in transgenic plants. Plant Cell, Tissue and Organ Culture 132 (1):1-25.

Kandel, R, D.R. Bergey, M. Dutt, V. Sitther, Z.T. Li, D. J. Gray and S.A. Dhekney. (2016). Evaluation of a Grapevine-derived Reporter Gene System for Precision Breeding of Vitis. Plant Cell Organ Tissue Culture. 124:599–609.

Dutt, M., L. Erpen, G. Ananthakrishnan, R.H. Brlansky, I. Maiti and J.W. Grosser. (2016). Comparative Expression Analysis of Five Caulimovirus Promoters in Citrus. Plant Cell Organ Tissue Culture 126:229–238.

Dutt, M., D. Stanton and J.W. Grosser. (2016). Ornacitrus: Development of Genetically Modified Anthocyanin Expressing Citrus with both Ornamental and Fresh Fruit Potential. Journal of the American Society for Horticultural Sciences 141:54-61.

Orbović, V., M. Ćalović, M. Dutt, J.W. Grosser and G. Barthe. (2015). Production and characterization of transgenic Citrus plants carrying p35 anti-apoptotic gene. Scientia Horticulturae 197:203-211.

Dutt M., G. Barthe, M. Irey, J. Grosser. (2015). Transgenic Citrus Expressing an Arabidopsis NPR1 Gene Exhibit Enhanced Resistance against Huanglongbing (HLB; Citrus Greening). PLoS ONE 10(9): e0137134.

Dutt, M., S.A. Dhekney, L. Soriano, R. Kandel, and J.W. Grosser. (2014). Temporal and spatial control of gene expression in horticultural crops. Horticulture Research 1:14047.

Bergey, D., R. Kandel, B. K. Tyree, M. Dutt and S. A. Dhekney. (2014). Role of Calmodulin and Related Proteins in Plant Cell Function: An Ever-Thickening Plot. Springer Science Reviews 2:145–159.

Grosser, J.W., D. Kainth and M. Dutt. (2014). Production of Colchicine Induced Autotetraploids in Pummelo (Citrus grandis) via Indirect Organogenesis. Hortscience 49 (7): 944-948.

Orbovic, V., M. Dutt, and J. W. Grosser. (2013). Evaluation of the germination potential of citrus seeds during the harvesting season. HortScience 48 (9): 1197-1199.

Shen, X., V. Orbovic, M. Dutt, W. S. Castle and F. G. Gmitter, Jr. (2013). Direct shoot organogenesis in Murraya paniculata (L.) Jack – a prerequisite for genetic transformation. HortScience 48(7): 938-41.

Dutt M., G. Ananthakrishnan, M.K. Jaromin, R.H. Brlansky and J.W. Grosser. (2012). Evaluation of four phloem-specific promoters in vegetative tissues of transgenic citrus plants. Tree Physiology 32(1):83-93.

Mondal, S. N., M. Dutt, J.W. Grosser and M. M. Dewdney. (2012). Transgenic citrus expressing the antimicrobial gene attacin E (attE) reduces the susceptibility of ‘Duncan’ grapefruit to the citrus scab caused by Elsinoë fawcettii. European Journal of Plant Pathology 133: 391-404.

Chae. C.W., M. Dutt, S. H. Yun, J. H. Park and D. H. Lee. (2011). Development of a SCAR marker linked to male fertility traits in ‘Jinkyool’(Citrus sunki). Journal of Life Science 21(12): 1659-1665.

Dutt M., M. Vasconcellos and J.W. Grosser. (2011). Effects of antioxidants on Agrobacterium-mediated transformation and accelerated production of transgenic plants of Mexican lime (Citrus aurantifolia Swingle). Plant Cell, Tissue and Organ Culture 107:79-89.

Orbovic, V., M. Dutt, and J. W. Grosser. (2011). Seasonal effects of fruit age on regeneration potential and transformation success rate in three citrus cultivars. Scientia Horticulturae 127: 262-266.

Dutt, M., D. H. Lee and J. W. Grosser. (2010). Bifunctional selection-reporter systems for genetic transformation of citrus: mannose and kanamycin based systems. In Vitro Cellular and Developmental Biology – Plant 46: 467-476.

Dutt, M. and J.W. Grosser. (2010). An embryogenic suspension cell culture system for Agrobacterium-mediated transformation of citrus. Plant Cell Reports 29:1251-1260.

Dutt M., M. Vasconcellos, K. Song, F. Gmitter, J.W.Grosser. (2010).  In vitro production of autotetraploid Ponkan mandarin (Citrus reticulata Blanco) using cell suspension cultures. Euphytica 173:235-242.

Dutt, M., J. Madhavaraj and J. W. Grosser. (2010). Agrobacterium tumefaciens-mediated genetic transformation and plant regeneration from a complex tetraploid hybrid citrus rootstock. Scientia Horticulturae 123:454-458.

Biswas, M. K., M. Dutt, U. K. Roy, R. Islam and M. Hossain. (2009). Development and evaluation of in vitro somaclonal variation in strawberry for improved horticultural traits. Scientia Horticulturae 122:409-416.

Dutt, M. and J.W. Grosser. (2009). Evaluation of parameters affecting Agrobacterium-mediated transformation of Citrus. Plant Cell, Tissue and Organ Culture 98: 331-340.

Dutt, M., Z.T. Li, S.A. Dhekney and D.J. Gray. (2008). A co-transformation system to produce transgenic grapevines free of marker genes. Plant Science 175 (3):423-430.

Geneve, R.L. and M. Dutt. (2008). Using sequential digital images to study seed germination and dormancy. Propagation of Ornamental Plants 8(1):13-16. 

Dhekney, S.A., Z.T. Li, M. Dutt and D.J. Gray. (2008).  Agrobacterium-mediated transformation of embryogenic cultures and plant regeneration in Vitis rotundifolia Michx. (Muscadine grape). Plant Cell Reports 27(5):865-72.

Li, Z.T., S.A. Dhekney, M. Dutt and D.J. Gray. (2008). An improved protocol for Agrobacterium-mediated transformation of grapevine. Plant Cell, Tissue and Organ Culture 93(3):311-321.

Dutt M., Z.T. Li, S.A. Dhekney and D.J. Gray. (2007). Transgenic plants from shoot apical meristems of Vitis vinifera L. “Thompson Seedless” via Agrobacterium-mediated transformation. Plant Cell Reports 26(12):2101-2110.

Dutt, M. and R. L. Geneve. (2007). Time to radicle protrusion does not correlate with early seedling growth in individual seeds of impatiens and petunia. Journal of the American Society for Horticultural Sciences 132: 423-428.

Li, Z. T., S. A. Dhekney, M. Dutt, M. Van Aman, J. Tattersall, K. T. Kelley, and D. J. Gray. (2006). Optimizing Agrobacterium-mediated transformation of grapevine. In Vitro Cellular and Developmental Biology – Plant 42(3): 220-227

Downie, A. B., L. M. A. Dirk, Q. Xu, J. Drake, M. Dutt, A. Levy, D. A. Butterfield, R. L. Geneve, J. W. Corum III, K. G. Lindstrom, and J. C. Snyder. (2004). A physical, enzymatic, and genetic characterization of perturbations in the brownseed mutants of tomato. Journal of Experimental Botany 55: 961-973.

Conference Proceedings

Dutt, M., G.A. Barthe, V. Orbovic M. Irey and J.W. Grosser (2013) Evaluation of transgenic citrus for disease resistance to HLB and Canker. Acta Horticulturae (submitted)

Dutt, M. and J.W. Grosser. 2011.  Agrobacterium Mediated Transformation of Citrus Cell Suspension Cultures. Acta Horticulturae 892:119-124. PDF

Grosser, J.W., M. Dutt, A. Omar, V. Orbovic, G. Barthe. 2011. Progress towards the development of transgenic disease resistance in citrus. Acta Horticulturae 892: 101-107. PDF

Dutt, M., A.A. Omar, V. Orbovic, G. Barthe and J.W. Grosser. 2010. Progress towards incorporation of antimicrobial peptides for disease resistance in citrus. Proceedings of the 11th International Citrus Congress pp. 258-264. PDF

Dutt, M., G. Barthe and J.W. Grosser. 2010. Systemic acquired resistance (SAR) in citrus: Genetic transformation with SAR genes to combat Huanglongbing and Canker. Proceedings of the 11th International Citrus Congress pp. 289-294. PDF

Dutt, M., V. Orbovic, and J.W. Grosser. 2009.  Cultivar dependent gene transfer into citrus using Agrobacterium. Proceedings of the Florida State Horticultural Society 122: 85 – 89. PDF

Dutt, M., A.A. Omar, V. Orbovic, G. Barthe, J. Gmitter, M. Vasconcellos, C. Dunning and J.W. Grosser. 2008. Combating Huanglongbing and Canker via Genetic Engineering of Citrus. Proceedings of the Florida State Horticultural Society 121:124-129. PDF

Geneve, R. L. and M. Dutt. 2008. A closer look at seed germination and dormancy. Combined Proceedings International Plant Propagator's Society 58: 561-565. PDF

Dutt, M., Z. T. Li, K. Kelley, S. A. Dhekney, M. Van Aman, J. Tattersall and D. J. Gray. 2007. Transgenic rootstock protein transmission in grapevines. Acta Horticulturae 738: 749-753. PDF

Li, Z. T., S. A. Dhekney, M. Dutt, M. Van Aman, J. Tattersall, K. Kelley and D. J. Gray. 2007.  Isolation and characterization of the 2S albumin gene and promoter from grapevine. Acta Horticulturae 738: 759-765. PDF

Dhekney, S. A., Z. T. Li., M. Van Aman, M. Dutt, J. Tattersall, and D. J. Gray. 2007. Genetic transformation of embryogenic cultures and recovery of transgenic plants in Vitis vinifera, Vitis rotundifolia and Vitis hybrids. Acta Horticulturae 738: 743 - 748. PDF

Dutt, M., S. T. Kester and R. L. Geneve. 2004. Elevated levels of ethylene during germination reduces the time to radicle emergence in impatiens. Acta Horticulturae 631: 43 – 47. PDF

Dutt, M. and R. L. Geneve. 2002. Use of computer imaging to evaluate the initial stages of germination in woody tree seeds. Combined Proceedings International Plant Propagator's Society 52: 484-488. PDF

Dutt, M. and R. L. Geneve. 2002. Using sequential digital images captured with a flat bed scanner to evaluate woody plant seeds with different germination requirements. Proceedings of Southern Nursery Association Research Conference 47: 352-356. PDF

Book Chapters

Dutt, M., C.A. El-Mohtar and N. Wang (2020) Biotechnological Approaches for the Resistance to Citrus Diseases. In: Gentile A., La Malfa S., Deng Z. (eds) The Citrus Genome. Compendium of Plant Genomes. Springer, pp 245-257

Germanà, M.A., P. Aleza, J.W. Grosser, M. Dutt, N. Wang, J. Cuenca and P. Kaur (2020) Chapter 9 - Citrus biotechnology. In: Talon M, Caruso M, Gmitter FG (eds) The Genus Citrus. Elsevier, Woodhead Publishing, pp 171-192. doi:

Dutt, M., Z.T. Li, S.A. Dhekney and D.J. Gray. 2012. Co-transformation of grapevine somatic embryos to produce transgenic plants free of marker genes In: J. Dunwell and A. Wetten (eds.).Transgenic Plants: Methods and Protocols. Springer New York pp. 201-213.

Dhekney, S.A., Z.T. Li, M. Dutt and D.J. Gray. 2012. Initiation and transformation of grapevine embryogenic cultures. In: J. Dunwell and A. Wetten (eds.).Transgenic Plants: Methods and Protocols. Springer New York pp. 215-225.