Publications - Dr. Nian Wang
Refereed Publications
2024
UF/IFAS Citrus Extension: Dr. Nian Wang
Su H, Wang Y, Xu J, Omar AA, Grosser JW, Wang N. Cas12a RNP-mediated co-transformation enables transgene-free multiplex genome editing, long deletions, and inversions in citrus chromosome. Front Plant Sci. 2024 Aug 1;15:1448807. https://doi.org/10.3389/fpls.2024.1448807
Lombardo MF, Zhang Y, Xu J, Trivedi P, Zhang P, Riera N, Li L, Wang Y, Liu X, Fan G, Tang J, Coletta-Filho HD, Cubero J, Deng X, Ancona V, Lu Z, Zhong B, Roper MC, Capote N, Catara V, Pietersen G, Al-Sadi AM, Xu X, Wang J, Yang H, Jin T, Cirvilleri G, Wang N. Global citrus root microbiota unravels assembly cues and core members. Front Microbiol. 2024 Jul 26;15:1405751. https://doi.org/10.3389/fmicb.2024.1405751
Wang N, Sundin GW, Fuente L, Cubero J, Tatineni S, Brewer MT, Zeng Q, Bock CH, Cunniffe NJ, Wang C, Candresse T, Chappell T, Coleman JJ, Munkvold G. Key Challenges in Plant Pathology in the Next Decade. Phytopathology. 2024 May;114(5):837-842. https://doi.org/10.1094/PHYTO-04-24-0137-KC
Khan A, Švara A, Wang N. Comparing Apples and Oranges: Advances in Disease Resistance Breeding of Woody Perennial Fruit Crops. Annu Rev Phytopathol. 2024 Sep;62(1):263-287. https://doi.org/10.1146/annurev-phyto-021622-120124
Jia H, Omar AA, Xu J, Dalmendray J, Wang Y, Feng Y, Wang W, Hu Z, Grosser JW, Wang N. Generation of transgene-free canker-resistant Citrus sinensis Hamlin in the T0 generation through Cas12a/CBE co-editing. Front Plant Sci. 2024 Mar 26;15:1385768. https://doi.org/10.3389/fpls.2024.1385768
Dai W, Pan M, Peng L, Zhang D, Ma Y, Wang M, Wang N. Integrated Transcriptome and Metabolome Analysis Reveals Insights into Flavone and Flavonol Biosynthesis in Salicylic Acid-Induced CitrusHuanglongbing Tolerance. J Agric Food Chem. 2024 Dec 26. https://doi.org/10.1021/acs.jafc.4c08160.
Huang G, Chang X, Hu Y, Li F, Wang N, Li R. SDE19, a SEC-dependent effector from 'Candidatus Liberibacter asiaticus' suppresses plant immunity and targets Citrus sinensis Sec12 to interfere with vesicle trafficking. PLoS Pathog. 2024 Sep 10;20(9):e1012542. https://doi.org/10.1371/journal.ppat.1012542
Lima HS, Mancine N, Peruchi GB, Francisco CS, Wang N, de Souza RSC, Armanhi JSL, Coletta-Filho HD. Microbial community of cultivated and uncultivated citrus rhizosphere microbiota in Brazil. Sci Data. 2024 Nov 27;11(1):1294. https://doi.org/10.1038/s41597-024-04141-y
Liu C, Li T, Cui L, Wang N, Huang G, Li R. OrangeExpDB: an integrative gene expression database for Citrus spp. BMC Genomics. 2024 May 27;25(1):521. https://doi.org/10.1186/s12864-024-10445-5.
Pena MM, Martins TZ, Teper D, Zamuner C, Alves HA, Ferreira H, Wang N, Ferro MIT, Ferro JA. EnvC Homolog Encoded by Xanthomonas citricitri Is Necessary for Cell Division and Virulence. Microorganisms. 2024 Mar 29;12(4):691. https://doi.org/10.3390/microorganisms12040691
Timilsina S, Kaur A, Sharma A, Ramamoorthy S, Vallad GE, Wang N, White FF, Potnis N, Goss EM, Jones JB. Xanthomonasas a Model System for Studying Pathogen Emergence and Evolution. Phytopathology. 2024 Jul;114(7):1433-1446. https://doi.org/10.1094/PHYTO-03-24-0084-RVW
Xiong Z, Yin H, Wang N, Han G, Gao Y. Chromosome-level genome assembly of navel orange cv. Gannanzao (Citrus sinensis Osbeck cv. Gannanzao). G3 (Bethesda). 2024 Feb 7;14(2):jkad268. https://doi.org/10.1093/g3journal/jkad268
Pandey SS, Li J, Oswalt C, Wang N. Dynamics of 'Candidatus Liberibacter asiaticus' Growth, Concentrations of Reactive Oxygen Species, and Ion Leakage in Huanglongbing-Positive Sweet Orange. Phytopathology. 2024 May;114(5):961-970. https://doi.org/10.1094/PHYTO-08-23-0294-KC
2023
UF/IFAS Citrus Extension: Dr. Nian Wang
Su H, Wang Y, Xu J, Omar AA, Grosser JW, Calovic M, Zhang L, Feng Y, Vakulskas CA, Wang N. Generation of the transgene-free canker-resistant Citrus sinensis using Cas12a/crRNA ribonucleoprotein in the T0 generation. Nature Commun. 2023 Jul 5;14(1):3957. https://doi.org/10.1038/s41467-023-39714-9
Huang X, Jia H, Xu J, Wang Y, Wen J, Wang N. Transgene-free genome editing of vegetatively propagated and perennial plant species in the T0 generation via a co-editing strategy. Nature Plants. 2023 Oct;9(10):1591-1597. https://doi.org/10.1038/s41477-023-01520-y
Carter EW, Peraza OG, Wang N. The protein interactome of the citrus Huanglongbing pathogen Candidatus Liberibacter asiaticus. Nature Commun. 2023 Nov 29;14(1):7838. https://doi.org/10.1038/s41467-023-43648-7
Xu J, Zhang Y, Li J, Teper D, Sun X, Jones D, Wang Y, Tao J, Goss EM, Jones JB, Wang N. Phylogenomic analysis of 343 Xanthomonas citri pv. citri strains unravels introduction history and dispersal paths. PLoS Pathog. 2023 Dec 15;19(12):e1011876. https://doi.org/10.1371/journal.ppat.1011876
Xiong Z, Yin H, Wang N, Han G, Gao Y. Chromosome-Level Genome Assembly of Navel orange cv. Gannanzao (Citrus sinensis Osbeck cv. Gannanzao). G3 (Bethesda). 2023 Nov 24:jkad268. https://doi.org/10.1093/g3journal/jkad268. Epub ahead of print.
Gao Y, Xu J, Li Z, Zhang Y, Riera N, Xiong Z, Ouyang Z, Liu X, Lu Z, Seymour D, Zhong B, Wang N. Citrus genomic resources unravel putative genetic determinants of Huanglongbing pathogenicity. iScience. 2023 Jan 23;26(2):106024. https://doi.org/10.1016/j.isci.2023.106024.
Teper D, White FF, Wang N. The Dynamic Transcription Activator-Like Effector Family of Xanthomonas. Phytopathology. 2023 Apr;113(4):651-666. https://doi.org/10.1094/PHYTO-10-22-0365-KD
Pandey SS, Xu J, Achor DS, Li J, Wang N. Microscopic and Transcriptomic Analyses of Early Events Triggered by 'CandidatusLiberibacter asiaticus' in Young Flushes of Huanglongbing-Positive Citrus Trees. Phytopathology. 2023 Jun;113(6):985-997. https://doi.org/10.1094/PHYTO-10-22-0360-R
Ribeiro C, Xu J, Hendrich C, Pandey SS, Yu Q, Gmitter FG Jr, Wang N. Seasonal Transcriptome Profiling of Susceptible and Tolerant Citrus Cultivars to Citrus Huanglongbing. Phytopathology. 2023 Feb;113(2):286-298. https://doi.org/10.1094/PHYTO-05-22-0179-R
Wang N, Scherm H. Key Discoveries in Plant Pathology During the Past Half Century: Impacts on the Life Sciences and on Plant Disease Management. Phytopathology. 2023 Apr;113(4):588-593. https://doi.org/10.1094/PHYTO-02-23-0070-KD
Khan A, Ben-David R, Richards J, Bansal U, Wang C, McCartney C, Stam R, Wang N. Plant Disease Resistance Research at the Dawn of the New Era. Phytopathology. 2023 May;113(5):756-759. https://doi.org/10.1094/PHYTO-03-23-0108-FI
de Souza-Neto RR, Vasconcelos FNDC, Teper D, Carvalho IGB, Takita MA, Benedetti CE, Wang N, de Souza AA. The Expansin Gene CsLIEXP1 Is a Direct Target of CsLOB1 in Citrus. Phytopathology. 2023 Jul;113(7):1266-1277. https://doi.org/10.1094/PHYTO-11-22-0424-R
2022
UF/IFAS Citrus Extension: Dr. Nian Wang
Ma W, Pang Z, Huang X, Xu J, Pandey SS, Li J, Achor DS, Vasconcelos FNC, Hendrich C, Huang Y, Wang W, Lee D, Stanton D, Wang N. Citrus Huanglongbing is a pathogen-triggered immune disease that can be mitigated with antioxidants and gibberellin. Nature Communications 2022 Jan 26;13(1):529. https://doi.org/10.1038/s41467-022-28189-9
Huang X, Wang Y, Wang N. 2022 Highly Efficient Generation of Canker-Resistant Sweet Orange Enabled by an Improved CRISPR/Cas9 System. Front Plant Sci. 12:769907. https://doi.org/10.3389/fpls.2021.769907
Huang X, Wang Y, Wang N. Base editors for citrus gene editing. bioRxiv 2021.10.19.464826; doi: https://doi.org/10.1101/2021.10.19.464826. (Note: This manuscript has been accepted for publication by Frontiers in Genome Editing).
Jia H, Omar A, Orbović V, Wang N. Biallelic editing of the LOB1 promoter via CRISPR/Cas9 creates canker-resistant 'Duncan' grapefruit. Phytopathology. 2021 Jul 2;. doi: https://doi.org/10.1094/PHYTO-04-21-0144-R.
Teper D, Xu J, Pandey SS, Wang N. PthAW1, a Transcription Activator-Like Effector of Xanthomonas citri citri, Promotes Host-Specific Immune Responses. Mol Plant Microbe Interact. 2021 Sep;34(9):1033-1047. doi: https://doi.org/10.1094/MPMI-01-21-0026-R.
Jia H, Wang Y, Su H, Huang X, Wang N. LbCas12a-D156R Efficiently Edits LOB1 Effector Binding Elements to Generate Canker-Resistant Citrus Plants. Cells. 2022; 11(3):315. https://doi.org/10.3390/cells11030315
2021
UF/IFAS Citrus Extension: Dr. Nian Wang
Hu B, Rao MJ, Deng X, Pandey SS, Hendrich C, Ding F, Wang N, Xu Q. Molecular signatures between citrus and Candidatus Liberibacter asiaticus. PLoS Pathog. 2021 Dec;17(12):e1010071. doi: https://doi.org/10.1371/journal.ppat.1010071.
Zhang Y, Andrade MO, Wang W, Teper D, Romeo T, Wang N. Examination of the Global Regulon of CsrA in Xanthomonas citri citri Using Quantitative Proteomics and Other Approaches. Mol Plant Microbe Interact. 2021 Nov;34(11):1236-1249. https://doi.org/10.1094/MPMI-05-21-0113-R.
Pandey SS, Hendrich C, Andrade M, Wang N. Candidatus Liberibacter: From Movement, Host Responses, to Symptom Development of Citrus HLB. Phytopathology. 2021 Oct 5;. https://doi.org/10.1094/PHYTO-08-21-0354-FI
Duan S, Long Y, Cheng S, Li J, Ouyang Z, Wang N. Rapid evaluation of the resistance of citrus germplasms against Xanthomonas citri subsp. citri. Phytopathology. 2021 Sep 8;. https://doi.org/10.1094/PHYTO-04-21-0175-R
Wang, N. 2021. A promising plant defense peptide against citrus Huanglongbing disease. Proc Natl Acad Sci U S A 118. 9;118:e2026483118 doi: https://doi.org/10.1073/pnas.2026483118.
Teper D, Wang N. 2021. Consequences of adaptation of TAL effectors on host susceptibility to Xanthomonas. PLoS Genet. 17(1):e1009310. doi: https://doi.org/10.1371/journal.pgen.1009310.
Ribeiro, C., Xu, J., Teper, D., Lee, D., and Wang, N. 2021. The transcriptome landscapes of citrus leaf in different developmental stages. Plant Molecular Biology. doi: https://doi.org/10.1007/s11103-021-01154-8.
Yuan, X., Chen, C., Bassanezi, R., Wu, F., Feng, Z., Shi, D., Du, Y., Zhong, L., Zhong, B., Lu, Z., Li, J., Song, X., Hu, Y., Ouyang, Z., Liu, X., Xie, J., Rao, X., Wang, X., Wu, D., Guan, Z., and Wang, N. 2021. Region-wide comprehensive implementation of roguing infected trees, tree replacement, and insecticide applications successfully controls citrus HLB. Phytopathology. https://doi.org/10.1094/PHYTO-09-20-0436-R.
Teper D, Pandey SS, Wang N. 2021. The HrpG/HrpX Regulon of Xanthomonads-An Insight to the Complexity of Regulation of Virulence Traits in Phytopathogenic Bacteria. Microorganisms. 9:187. doi: https://doi.org/10.3390/microorganisms9010187.
Li, J., Kolbasov, V., Lee, D., Pang, Z., Yixiao, H., Collins, N., and Wang, N. 2021. Residue dynamics of streptomycin in citrus delivered by foliar spray and trunk injection and effect on 'Candidatus Liberibacter asiaticus' titer. Phytopathology. doi: https://doi.org/10.1094/PHYTO-09-20-0427-R.
Vasconcelos, F N C., Li, J., Pang, Z., Vincent, C., and Wang, N. 2021. Census of Candidatus Liberibacter asiaticus population inside the phloem of citrus trees. Phytopathology. doi: https://doi.org/10.1094/PHYTO-09-20-0388-R.
Pandey, S.S., F, N.C.V., and Wang, N. 2021. Spatiotemporal dynamics of Candidatus Liberibacter asiaticus colonization inside citrus plant and Huanglongbing disease development. Phytopathology. doi: https://doi.org/10.1094/PHYTO-09-20-0407-R.
Li, J., Kolbasov, V.G., Pang, Z., Duan, S., Lee, D., Huang, Y., Xu, J., Teper, D., Lamichhane, T., and Wang, N. 2021. Evaluation of the control effect of SAR inducers against citrus Huanglongbing applied by foliar spray, soil drench or trunk injection. Phytopathology Research 3:2 doi: https://doi.org/10.1186/s42483-020-00079-2
2020
UF/IFAS Citrus Extension: Dr. Nian Wang
Pang, Z., Zhang, L., Coaker, G., Ma, W., He, S.Y., and Wang, N. 2020. Citrus CsACD2 Is a Target of Candidatus Liberibacter Asiaticus in Huanglongbing Disease. Plant Physiol 184:792-805.
Teper, D., Xu, J., Li, J., and Wang, N. 2020. The immunity of Meiwa kumquat against Xanthomonas citri is associated with a known susceptibility gene induced by a transcription activator-like effector. PLoS Pathog 16:e1008886.
Andrade, M., Li, J., and Wang, N. 2020a. Candidatus Liberibacter asiaticus: virulence traits and control strategies. Tropical Plant Pathology.
Chen, T., Nomura, K., Wang, X., Sohrabi, R., Xu, J., Yao, L., Paasch, B.C., Ma, L., Kremer, J., Cheng, Y., Zhang, L., Wang, N., Wang, E., Xin, X.F., and He, S.Y. 2020b. A plant genetic network for preventing dysbiosis in the phyllosphere. Nature 580:653-657.
Huang, X., Wang, Y., Xu, J., and Wang, N. 2020. Development of multiplex genome editing toolkits for citrus with high efficacy in biallelic and homozygous mutations. Plant Mol Biol 104:297-307.
Jia, H., and Wang, N. 2020. Generation of homozygous canker-resistant citrus in the T0 generation using CRISPR-SpCas9p. Plant Biotechnol J. 18:1990-2
An, S.Q., Potnis, N., Dow, M., Vorhölter, F.J., He, Y.Q., Becker, A., Teper, D., Li, Y., Wang, N., Bleris, L., and Tang, J.L. 2020. Mechanistic insights into host adaptation, virulence and epidemiology of the phytopathogen Xanthomonas. FEMS Microbiol Rev 44:1-32.
Andrade, M.O., Pang, Z., Achor, D.S., Wang, H., Yao, T., Singer, B.H., and Wang, N. 2020b. The flagella of 'Candidatus Liberibacter asiaticus' and its movement in planta. Mol Plant Pathol 21:109-123.
Clark, K., Pang, Z., Trinh, J., Wang, N., and Ma, W. 2020. Sec-delivered effector 1 (SDE1) of Candidatus Liberibacter asiaticus promotes citrus Huanglongbing. Mol Plant Microbe Interact. (Accepted)
Franco, J., Thapa, S.P., Pang, Z., Gurung, F.B., Liebrand, T.W., Stevens, D.M., Ancona, V., Wang, N., and Coaker, G. 2020. Citrus vascular proteomics highlights the role of peroxidases and serine proteases during Huanglongbing disease progression. Mol Cell Proteomics.(Accepted)
Pena, M.M., Teper, D., Ferreira, H., Wang, N., Sato, K.U., Ferro, M.I.T., and Ferro, J.A. 2020. mCherry fusions enable the subcellular localization of periplasmic and cytoplasmic proteins in Xanthomonas sp. PLoS One 15:e0236185.
Qiu, W., Soares, J., Pang, Z., Huang, Y., Sun, Z., Wang, N., Grosser, J., and Dutt, M. 2020. Potential Mechanisms of AtNPR1 Mediated Resistance against Huanglongbing (HLB) in Citrus. Int J Mol Sci 21.
Thapa, S.P., De Francesco, A., Trinh, J., Gurung, F.B., Pang, Z., Vidalakis, G., Wang, N., Ancona, V., Ma, W., and Coaker, G. 2020. Genome-wide analyses of Liberibacter species provides insights into evolution, phylogenetic relationships, and virulence factors. Mol Plant Pathol.
Wang, N. 2020. A perspective of citrus Huanglongbing in the context of the Mediterranean Basin. Journal of Plant Pathology.
Zhang, Y., Xu, J., Wang, E., and Wang, N. 2020. Mechanisms Underlying the Rhizosphere-To-Rhizoplane Enrichment of Cellvibrio Unveiled by Genome-Centric Metagenomics and Metatranscriptomics. Microorganisms 8.
2019
UF/IFAS Citrus Extension: Dr. Nian Wang
Patané JSL, Martins J Jr, Rangel LT, Belasque J, Digiampietri LA, Facincani AP, Ferreira RM, Jaciani FJ, Zhang Y, Varani AM, Almeida NF, Wang N, Ferro JA, Moreira LM, Setubal JC. 2019 Origin and diversification of Xanthomonas citri subsp. citri pathotypes revealed by inclusive phylogenomic, dating, and biogeographic analyses. BMC Genomics. 20:700. https://doi.org/10.1186/s12864-019-6007-4
Zhang, Y. , Teper, D., Xu, J., and Wang, N. Stringent response regulators (p)ppGpp and DksA positively regulate virulence and host adaptation of Xanthomonas citri. Mol Plant Pathol 20:1550-1565. https://doi.org/10.1111/mpp.12865
Li, J., Pang, Z., Duan, S., Lee, D., Kolbasov, V., and Wang, N. The in planta effective concentration of oxytetracycline against Candidatus Liberibacter asiaticus for suppression of citrus Huanglongbing. Phytopathology. 109:2046-2054. https://doi.org/10.1094/PHYTO-06-19-0198-R
Nian Wang. 2019. The citrus Huanglongbing crisis and potential solutions. Molecular Plant. 12: 607-609. https://doi.org/10.1016/j.molp.2019.03.008.
Maxuel O. Andrade and Nian Wang. 2019. The Tad pilus apparatus of Candidatus Liberibacter asiaticus and its regulation by VisNR. Molecular Plant-Microbe Interactions. 32:1175-1187. http://doi.org/10.1094/MPMI-02-19-0052-R
Hongge Jia, Vladimir Orbović, Nian Wang. CRISPR‐LbCas12a‐mediated modification of citrus. 2019. Plant Biotechnol J. 17: 1928-1937 https://doi.org/10.1111/pbi.13109.
Doron Teper, Yanan Zhang, Nian Wang. 2019. TfmR, a novel TetR‐family transcriptional regulator, modulates the virulence of Xanthomonas citri in response to fatty acids. Mol Plant Pathol. 20:701-715 https://doi.org/10.1111/mpp.12786.
Qibin Yu, Ming Huang1, Hongge Jia, Yuan Yu, Anne Plotto, Elizabeth A. Baldwin, Jinhe Bai, Nian Wang and Frederick G. Gmitter Jr. 2019. Deficiency of valencene in mandarin hybrids is associated with a deletion in the promoter region of the valencene synthase gene. BMC Plant Biology 19:101. https://doi.org/10.1186/s12870-019-1701-6
Lei Li, Jinyun Li, Yunzeng Zhang and Nian Wang. 2019. Diffusible signal factor (DSF)-mediated quorum sensing modulates expression of diverse traits in Xanthomonas citri and responses of citrus plants to promote disease. BMC Genomics 20:55. https://doi.org/10.1186/s12864-018-5384-4
Muhammad Junaid Rao, Fang Ding, Nian Wang, Xiuxin Deng & Qiang Xu. 2019. Metabolic Mechanisms of Host Species Against Citrus Huanglongbing (Greening Disease). Critical Reviews in Plant Sciences, 37:6, 496-51. https://doi.org/10.1080/07352689.2018.1544843
Christopher Vincent, Myrtho Pierre, Jinyun Li and Nian Wang. 2019. Implications of Heat Treatment and Systemic Delivery of Foliar-Applied Oxytetracycline on Citrus Physiological Management and Therapy Delivery. Front. Plant Sci. 10:41. https://doi.org/10.3389/fpls.2019.00041
Sheo Shankar Pandey and Nian Wang. 2019. Targeted early detection of citrus HLB causal agent ‘Candidatus Liberibacter asiaticus’ before symptom expression. Phytopathology. 109:952-959. https://doi.org/10.1094/PHYTO-11-18-0432-R
Li J, Li L, Pang Z, Kolbasov V, Ehsani R, Carter E, Wang N. 2019 Developing citrus Huanglongbing management strategies based on the severity of symptoms in HLB-endemic citrus-producing regions. Phytopathology. 109:582-592. https://doi.org/10.1094/PHYTO-08-18-0287-R.
Xu J, Wang N. 2019 Where are we going with genomics in plant pathogenic bacteria? Genomics.111:729-736. https://doi.org/10.1016/j.ygeno.2018.04.011
2018
UF/IFAS Citrus Extension: Dr. Nian Wang
Xu J, Zhang Y, Zhang P, Trivedi P, Riera N, Wang Y, Liu X, Fan G, Tang J, Coletta-Filho HD, Cubero J, Deng X, Ancona V, Lu Z, Zhong B, Roper MC, Capote N, Catara V, Pietersen G, Vernière C, Al-Sadi AM, Li L, Yang F, Xu X, Wang J, Yang H, Jin T, Wang N. 2018 The structure and function of the global citrus rhizosphere microbiome. Nat Commun. 20;9(1):4894. https://doi.org/10.1038/s41467-018-07343-2
Hu, J., Jiang, J., and Wang, N. 2018. Control of Citrus Huanglongbing via Trunk Injection of Plant Defense Activators and Antibiotics. Phytopathology 108:186-195. https://doi.org/10.1094/PHYTO-05-17-0175-R
Zhou, X., Teper, D., Andrade, M.O., Zhang, T., Chen, S., Song, W.Y., and Wang, N. 2018. A Phosphorylation Switch on Lon Protease Regulates Bacterial Type III Secretion System in Host. MBio 9. https://doi.org/10.1128/mBio.02146-17
Sundin, G.W., and Wang, N. 2018. Antibiotic Resistance in Plant-Pathogenic Bacteria. Annu Rev Phytopathol. https://doi.org/10.1146/annurev-phyto-080417-045946. [Epub ahead of print].
Clark, K., Franco, J.Y., Schwizer, S., Pang, Z., Hawara, E., Liebrand, T.W.H., Pagliaccia, D., Zeng, L., Gurung, F.B., Wang, P., Shi, J., Wang, Y., Ancona, V., van der Hoorn, R.A.L., Wang, N., Coaker, G., and Ma, W. 2018. An effector from the Huanglongbing-associated pathogen targets citrus proteases. Nat Commun 9:1718. https://doi.org/10.1038/s41467-018-04140-9
Duan, S., Jia, H., Pang, Z., Teper, D., White, F., Jones, J., Zhou, C., and Wang, N. 2018. Functional characterization of the citrus canker susceptibility gene CsLOB1. Mol Plant Pathol. doi: 10.1111/mpp.12667. https://doi.org/10.1111/mpp.12667 [Epub ahead of print]
Ference, C.M., Gochez, A.M., Behlau, F., Wang, N., Graham, J.H., and Jones, J.B. 2018. Recent advances in the understanding of Xanthomonas citri ssp. citri pathogenesis and citrus canker disease management. Mol Plant Pathol 19:1302-1318. https://doi.org/10.1111/mpp.12638
Gochez, A.M., Huguet-Tapia, J.C., Minsavage, G.V., Shantaraj, D., Jalan, N., Strauß, A., Lahaye, T., Wang, N., Canteros, B.I., Jones, J.B., and Potnis, N. 2018. Pacbio sequencing of copper-tolerant Xanthomonas citri reveals presence of a chimeric plasmid structure and provides insights into reassortment and shuffling of transcription activator-like effectors among X. citri strains. BMC Genomics 19:16. https://doi.org/10.1186/s12864-017-4408-9
Riera, N., Wang, H., Li, Y., Li, J., Pelz-Stelinski, K., and Wang, N. 2018. Induced systemic resistance against citrus canker disease by rhizospheric bacteria. Phytopathology. doi.org/10.1094/PHYTO-07-17-0244-R https://doi.org/10.1094/PHYTO-07-17-0244-R
Xu, J., and Wang, N. 2018. Where are we going with genomics in plant pathogenic bacteria? Genomics. https://doi.org/10.1016/j.ygeno.2018.04.011
2017
UF/IFAS Citrus Extension: Dr. Nian Wang
Jia, H., Xu, J., Orbović, V., Zhang, Y., and Wang, N. 2017. Editing Citrus Genome via SaCas9/sgRNA System. Front Plant Sci 8:2135. https://doi.org/10.3389/fpls.2017.02135
Jin, T., Wang, Y., Huang, Y., Xu, J., Zhang, P., Wang, N., Liu, X., Chu, H., Liu, G., Jiang, H., Li, Y., Kristiansen, K., Xiao, L., Zhang, Y., Zhang, G., Du, G., Zhang, H., Zou, H., Jie, Z., Liang, S., Jia, H., Wan, J., Lin, D., Li, J., Fan, G., Yang, H., Wang, J., Bai, Y., and Xu, X. 2017. Taxonomic structure and functional association of foxtail millet root microbiome. Gigascience 6:1-12. https://doi.org/10.1093/gigascience/gix089
Pagliaccia, D., Shi, J., Pang, Z., Hawara, E., Clark, K., Thapa, S.P., De Francesco, A.D., Liu, J., Tran, T.T., Bodaghi, S., Folimonova, S.Y., Ancona, V., Mulchandani, A., Coaker, G., Wang, N., Vidalakis, G., and Ma, W. 2017. A Pathogen Secreted Protein as a Detection Marker for Citrus Huanglongbing. Front Microbiol 8:2041. https://doi.org/10.3389/fmicb.2017.02041
Riera, N., Handique, U., Zhang, Y., Dewdney, M.M., and Wang, N. 2017. Characterization of Antimicrobial-Producing Beneficial Bacteria Isolated from Huanglongbing Escape Citrus Trees. Front Microbiol 8:2415. https://doi.org/10.3389/fmicb.2017.02415
Wang, N., Stelinski, L.L., Pelz-Stelinski, K.S., Graham, J.H., and Zhang, Y. 2017. Tale of the Huanglongbing Disease Pyramid in the Context of the Citrus Microbiome. Phytopathology 107:380-387. https://doi.org/10.1094/PHYTO-12-16-0426-RVW
Wang N, Pierson EA, Setubal JC, Xu J, Levy J G., Zhang Y, Li J, Rangel LT, and J., M. 2017. The Candidatus Liberibacter–Host Interface: Insights into Pathogenesis Mechanisms and Disease Control. Annual Review of Phytopathology. 55: 451-482. https://doi.org/10.1146/annurev-phyto-080516-035513
Zhang, J., Huguet-Tapia, J.C., Hu, Y., Jones, J., Wang, N., Liu, S., and White, F.F. 2017a. Homologues of CsLOB1 in citrus function as disease susceptibility genes in citrus canker. Mol Plant Pathol 18:798-810. https://doi.org/10.1111/mpp.12441
Zhang, Y., Xu, J., Riera, N., Jin, T., Li, J., and Wang, N. 2017b. Huanglongbing impairs the rhizosphere-to-rhizoplane enrichment process of the citrus root-associated microbiome. Microbiome 5:97. https://doi.org/10.1186/s40168-017-0304-4
Li J., Pang, Z.p, Trivedi Pp, Zhou Xg, Ying Xp, Jia Hp, and Wang N. 2017 'Candidatus Liberibacter asiaticus' Encodes a Functional Salicylic Acid (SA) Hydroxylase that Degrades SA to Suppress Plant Defenses. Mol Plant Microbe Interact30:620-630. https://doi.org/10.1094/MPMI-12-16-0257-R
Zhou X, Yan Qp, Wang N. 2017 Deciphering the regulon of a GntR family regulator via transcriptome and ChIP-exo analyses and its contribution to virulence in Xanthomonas citri. Mol Plant Pathol. 18: 249-262. https://doi.org/10.1111/mpp.12397
Gochez AM, Shantharaj D, Potnis N, Zhou X, Minsavage GV, White FF, Wang N, Hurlbert JC, Jones JB 2017 Molecular characterization of XopAG Effector-AvrGf2 from Xanthomonas fuscans subsp. aurantifolii in grapefruit Molecular Plant Pathology. 18: 405-419. https://doi.org/10.1111/mpp.12408
Jia H, Zhang Y, Orbovic V, Xu J, White F, Jones J, Wang N. (2017) Genome editing of the disease susceptibility gene CsLOB1 in citrus confers resistance to citrus canker. Plant Biotechnol J. 15 (7), 817-823. https://doi.org/10.1111/pbi.12677
2016
Samiksha Prasad, Jin Xu, Yunzeng Zhang, and Nian Wang (2016) SEC-Translocon Dependent Extracytoplasmic Proteins of Candidatus Liberibacter asiaticus. Frontiers in Microbiology. 7:1989. https://doi.org/10.3389/fmicb.2016.01989
Zhang Y, Barthe G, Grosser JW, Wang N. (2016) Transcriptome analysis of root response to citrus blight based on the newly assembled Swingle citrumelo draft genome. BMC Genomics. 17:485. https://doi.org/10.1186/s12864-016-2779-y
Zhang J, Huguet-Tapia JC, Hu Y, Jones J, Wang N, Liu S, White FF. (2016) Homologues of CsLOB1 in citrus function as disease susceptibility genes in citrus canker. Mol Plant Pathol. 2016 Jun 8. https://doi.org/10.1111/mpp.12441
Sundin GW, Wang N, Charkowski AO, Castiblanco LF, Jia H, Zhao Y. (2016) Perspectives on the Transition From Bacterial Phytopathogen Genomics Studies to Applications Enhancing Disease Management: From Promise to Practice. Phytopathology.106:1071-1082. https://doi.org/10.1094/PHYTO-03-16-0117-FI
Hu J, Wang N. (2016) Evaluation of the Spatiotemporal Dynamics of Oxytetracycline and Its Control Effect Against Citrus Huanglongbing via Trunk Injection. Phytopathology. 106:1495-1503. https://doi.org/10.1094/PHYTO-02-16-0114-R
Hu J, Akula N, Wang N (2016) Development of a Microemulsion Formulation for Antimicrobial SecA Inhibitors. PLoS ONE 11(3): e0150433. doi:10.1371/journal.pone.0150433. https://doi.org/10.1371/journal.pone.0150433
Jia, H., Orbovic, V., Jones, J.B. and Wang, N. 2016 Modification of the PthA4 effector binding elements in Type I CsLOB1 promoter using Cas9/sgRNA to produce transgenic Duncan grapefruit alleviating XccΔpthA4:dCsLOB1.3 infection. Plant Biotechnol. J., 14: 1291–1301. https://doi.org/10.1111/pbi.12495
Laís Moreira Granato; Simone Cristina Picchi; Maxuel de Oliveira Andrade; Marco Aurélio Takita; Alessandra Alves de Souza; Nian Wang; Marcos Antonio Machado ATP-dependent RNA helicase HrpB plays an important role in motility and biofilm formation in Xanthomonas citri subsp citri. BMC Microbiology. 16:55. https://doi.org/10.1186/s12866-016-0655-1
Yang Hu, Shuo Duan, Yunzeng Zhang, Deepak Shantharaj, Jeffrey B. Jones, and Nian Wang 2016 Temporal transcription profiling of sweet orange in response to PthA4-mediated Xanthomonas citri subsp. citri infection Phytopathology 106:442-51. https://doi.org/10.1094/PHYTO-09-15-0201-R
Li, J., Trivedi, P., and Wang, N. 2016. Field Evaluation of Plant Defense Inducers for the Control of Citrus Huanglongbing. Phytopathology: 106:37-46 https://doi.org/10.1094/PHYTO-08-15-0196-R
2015
Ravindran, A., Jalan, N., Yuan, J.S., Wang, N., and Gross, D.C. 2015. Comparative genomics of Pseudomonas syringae pv. syringae strains B301D and HS191 and insights into intrapathovar traits associated with plant pathogenesis. Microbiologyopen 4:553-573. https://doi.org/10.1002/mbo3.261
Zhang, Y., Jalan, N., Zhou, X., Goss, E., Jones, J.B., Setubal, J.C., Deng, X., and Wang, N. 2015. Positive selection is the main driving force for evolution of citrus canker-causing Xanthomonas. ISME J. 9: 2128–2138. https://doi.org/10.1038/ismej.2015.15
Zhou, X., Hu, X., Li, J., and Wang, N. 2015. A Novel Periplasmic Protein, VrpA, Contributes to Efficient Protein Secretion by the Type III Secretion System in Xanthomonas spp. Mol Plant Microbe Interact 28:143-153. http://dx.doi.org/10.1094/MPMI-10-14-0309-R
Wang N, Jin T, Trivedi P, Setubal JC, Tang J, Machado MA, Triplett E, Coletta-Filho HD, Cubero J, Deng X, et al. 2015. Announcement of the International Citrus Microbiome (Phytobiome) Consortium. J Cit Pathol. iocv_journalcitruspathology_27940. https://escholarship.org/uc/item/5xp3v2rc
2014
Maxuel O. Andrade, Chuck S. Farah, Nian Wang. 2014 The post-transcriptional regulator rsmA/csrA activates T3SS by stabilizing the 5' UTR of hrpG, the master regulator of hrp/hrc genes, in Xanthomonas. PLoS Pathog 10(2): e1003945. doi:10.1371/journal.ppat.1003945. https://doi.org/10.1371/journal.ppat.1003945
Trivedi P., Wang N. 2014 Host immune responses accelerate pathogen evolution ISME J. 8, 727–731. https://doi.org/10.1038/ismej.2013.215
Yang Hu, Junli Zhang, Hongge Jia, Davide Sosso, Ting Li, Wolf B. Frommer, Bing Yang, Frank F. White, Nian Wang, and Jeffrey B. Jones 2014 Lateral organ boundaries 1 is a disease susceptibility gene for citrus bacterial canker disease Proc Natl Acad Sci U S A. 111(4):E521-9. https://doi.org/10.1073/pnas.1313271111
Jia H., Wang N. 2014 Targeted genome editing of sweet orange using Cas9/sgRNA. PLoS One. 9(4):e93806. https://doi.org/10.1371/journal.pone.0093806
Jia, H., and Wang, N. 2014b. Xcc-facilitated agroinfiltration of citrus leaves: a tool for rapid functional analysis of transgenes in citrus leaves. Plant Cell Rep 33:1993-2001. https://doi.org/10.1007/s00299-014-1673-9
Li J, Wang N. 2014 Foliar application of biofilm formation inhibiting compounds enhances control of citrus canker caused by Xanthomonas citri subsp. citri. Phytopathology 104: 134-142. https://doi.org/10.1094/PHYTO-04-13-0100-R
Sunitha Kogenaru, Qing Yan, Nadia Riera, M Caroline Roper, Xiaoling Deng, Timothy A Ebert, Michael Rogers, Michael E Irey, Gerhard Pietersen, Charles M Rush and Nian Wang. 2014 Repertoire of novel sequence signatures for the detection of Candidatus Liberibacter asiaticus by quantitative real-time PCR. BMC Microbiology 2014 14:39. https://doi.org/10.1186/1471-2180-14-39
2013
Wang, N.; Trivedi, P. 2013 Citrus Huanglongbing - an old problem with an unprecedented challenge. Phytopathology. 103: 652-665 (Invited review). https://doi.org/10.1094/PHYTO-12-12-0331-RVW
Aritua V, Achor D, Gmitter FG, Albrigo G, Wang N. 2013 Transcriptional and Microscopic Analyses of Citrus Stem and Root Responses to Candidatus Liberibacter asiaticus Infection. PLoS One. 8(9):e73742. doi: 10.1371/journal.pone.0073742. https://doi.org/10.1371/journal.pone.0073742
Jalan N, Kumar D, Andrade MO, Yu F, Jones JB, Graham JH, White FF, Setubal JC, Wang N. 2013 Comparative genomic and transcriptome analyses of pathotypes of Xanthomonas citri subsp. citri provide insights into mechanisms of bacterial virulence and host range. BMC Genomics. https://doi.org/10.1186/1471-2164-14-551
Qing Yan, Aswathy Sreedharan, Shiping Wei, Jihua Wang, Kirsten Pelz-Stelinski, Svetlana Folimonova, Nian Wang. 2013 Global gene expression changes in Candidatus Liberibacter asiaticus during the transmission in distinct hosts between plant and insect. Molecular Plant Pathology. 14:391-404. https://doi.org/10.1111/mpp.12015
Jalan N, Kumar D, Yu F, Jones JB, Graham JH, Wang N. Complete Genome Sequence of Xanthomonas citri subsp. citri Strain Aw12879, a Restricted-Host-Range Citrus Canker-Causing Bacterium. Genome Announc. 1(3). doi:pii: e00235-13. https://doi.org/10.1128/genomeA.00235-13
Xiuhua Chen, Jinyoung Y. Barnaby, Aswathy Sreedharan, Xiaoen Huang, Vladimir Orbović, Jude W. Grosser, Nian Wang, Xinnian Dong, Wen-Yuan Song 2013 Over-expression of the citrus gene CtNH1 confers resistance to bacterial canker disease. Physiological and Molecular Plant Pathology 84: 115–122. https://doi.org/10.1016/j.pmpp.2013.07.002
2012
Trivedi, P., He, Z., Van Nostrand, J. D., Zhou J., Albrigo, L. G., and Wang, N. 2012 Huanglongbing alters the structure and functional diversity of microbial communities associated with citrus rhizosphere. ISME J. 6, 363-383. https://doi.org/10.1038/ismej.2011.100
Kogenaru, S.,Yan, Q., Guo, Y., and Wang, N. 2012 RNA-seq and microarray complement each other in transcriptome profiling. BMC Genomics 13:629 (doi:10.1186/1471-2164-13-629). https://doi.org/10.1186/1471-2164-13-629
Wang, N, Li JL, Lindow SE. 2012 RpfF-dependent regulon of Xylella fastidiosa. Phytopathology. 102:1045-53. https://doi.org/10.1094/PHYTO-07-12-0146-R
Guo, Y., Zhang, Y., Li, J.L., Wang, N. 2012 DSF-mediated quorum sensing plays a central role in coordinating gene expression of Xanthomonas citri subsp. citri. Molecular Plant-Microbe Interactions 25: 165-179. https://doi.org/10.1094/MPMI-07-11-0184
Yan, Q., and Wang, N. 2012 High-throughput screening and analysis of genes of Xanthomonas citri subsp. citri involved in citrus canker symptom development Molecular Plant-Microbe Interactions 25:69-84. https://doi.org/10.1094/MPMI-05-11-0121
Qing Yan, Xiufang Hu, Nian Wang 2012 The novel virulence related gene nlxA is involved in the production of lipopolysaccharide and extracellular polysaccharide, motility, biofilm formation and stress resistance of Xanthomonas citri subsp. citri. Molecular Plant Pathology 13:923-34. https://doi.org/10.1111/j.1364-3703.2012.00800.x
Li J., and Wang, N. 2012 The gpsX gene encoding glycosyltransferase is important for polysaccharide production and required for full virulence in Xanthomonas citri subsp. citri BMC Microbiology 12: 31. https://doi.org/10.1186/1471-2180-12-31
Akula, N., Trivedi, P., Han, F.Q., Wang, N. 2012 Identification of small molecule inhibitors against SecA of Candidatus Liberibacter asiaticus by structure based design. European Journal of Medicinal Chemistry 54:919-24. https://doi.org/10.1016/j.ejmech.2012.05.035
2011
Neha Jalan, Valente Aritua, Dibyendu Kumar, Fahong Yu, Jeffrey B. Jones, James H. Graham, João C. Setubal, and Nian Wang. 2011 Comparative genomic analysis of Xanthomonas axonopodis pv. citrumelo F1 causing citrus bacterial spot and related strains provides insights into virulence and host-specificity. Journal of Bacteriology 193:6342-57. https://doi.org/10.1128/JB.05777-11
Li, J. and Wang, N. 2011b Genome-wide mutagenesis of Xanthomonas axonopodis pv. citri reveals novel genetic determinants and regulation mechanisms of biofilm formation. PLoS ONE 6(7): e21804. https://doi.org/10.1371/journal.pone.0021804
Guo, Y., Figueiredo, F., Jones, J., and Wang, N. 2011 HrpG, together with HrpX, play global roles in coordinating different virulence traits of Xanthomonas axonopodis pv. citri Molecular Plant-Microbe Interactions 12:381-96. https://doi.org/10.1094/ MPMI -09-10-0209
Trivedi, P. Spann, T. and Wang, N. 2011 Isolation and Characterization of Beneficial Bacterial Community Associated with Citrus Roots in Florida. Microbial Ecology 62: 324-36. https://doi.org/10.1007/s00248-011-9822-y
Akula N, Zheng H, Han FQ, Wang, N. 2011 Discovery of novel SecA inhibitors of Candidatus Liberibacter asiaticus by structure based design. Bioorg Med Chem Lett. 21:4183-8. https://doi.org/10.1016/j.bmcl.2011.05.086
Yan Q., and Wang, N. 2011a The ColR/ColS two-component system plays multiple roles in the pathogenicity of the citrus canker pathogen Xanthomonas citri subsp. citri Journal of Bacteriology 193:1590-1599. https://doi.org/10.1128/JB.01415-10
Li, J. and Wang, N. 2011 The wxacO gene of Xanthomonas citri ssp. citri encodes a protein with a role in lipopolysaccharide biosynthesis, biofilm formation, stress tolerance and virulence  Molecular Plant Pathology 12:381-396. https://doi.org/10.1111/j.1364-3703.2010.00681.x
2010
Guo, Y., Sagaram, U.S., Kim, J.S. and Wang, N. 2010. Requirement of the galU gene for polysaccharide production by and pathogenicity and growth in planta of Xanthomonas citri subsp. citri. Applied and Environmental Microbiology 76:2234‑2242. https://doi.org/10.1128/AEM.02897-09
Trivedi, P., Duan, Y., and Wang, N. 2010. Huanglongbing, a systemic disease, restructures the bacterial community associated with citrus roots. Applied and Environmental Microbiology 76: 3427–3436. https://doi.org/10.1128/AEM.02901-09
2009
Sagaram, U.S., DeAngelis, K.M., Trivedi, P., Andersen, G.L., Lu, S., and Wang, N. 2009. Bacterial diversity analysis of Huanglongbing pathogen-infected citrus using PhyloChips and 16S rDNA clone library sequencing. Applied and Environmental Microbiology 75:1566-1574. https://doi.org/10.1128/AEM.02404-08
Kim, J., Sagaram, U.S., Burns, J.K., and Wang, N. 2009. Response of sweet orange (Citrus sinensis) to Candidatus Liberibacter asiaticus infection: microscopy and microarray analyses. Phytopathology 99:50-57. https://doi.org/10.1094/PHYTO-99-1-0050
Trivedi, P., Sagaram, U.S., Kim, J.S., Brlansky, R.H., Rogers, M., Stelinski, L.L., Oswalt, C., Kim, J.S., and Wang, N. 2009. Quantification of viable Candidatus Liberibacter asiaticus in hosts using quantitative PCR with the aid of ethidium monoazide (EMA). European Journal of Plant Pathology 124:553-563. https://doi.org/10.1007/s10658-009-9439-x
Gu, G., Smith, L., Wang, N., Wang, H., and Lu, S. 2009a. Biosynthesis of an antifungal oligopeptide in Burkholderia contaminans strain MS14. Biochemical and Biophysical Research Communications 380:328-332. https://doi.org/10.1016/j.bbrc.2009.01.073
Gu, G., Wang, N., Chaney, N., Smith, L., and Lu, S. 2009b. AmbR1 is a key transcriptional regulator for production of antifungal activity of Burkholderia contaminans strain MS14. FEMS Microbiological Letters 297:54-60. https://doi.org/10.1111/j.1574-6968.2009.01653.x
Boina, D., Rogers, M.E., Wang, N., and Stelinski, L.L. 2009. Effect of pyriproxyfen, a juvenile hormone mimic, on egg hatch, nymph development, adult emergence and reproduction of the Asian citrus psyllid, Diaphorina citri Kuwayama. Pest Management Science 66: 349-357. https://doi.org/10.1002/ps.1880
2008
Tatineni, S., Sagaram, U.S., Gowda, S., Robertson, C.J., Dawson, W.O., Iwanami, T., and Wang, N. 2008. In planta distribution of 'Candidatus Liberibacter asiaticus' as revealed by polymerase chain reaction (PCR) and real-time PCR. Phytopathology 98(5):592‑599. https://doi.org/10.1094/PHYTO-98-5-0592
2006
Wang, N., Lu, S.E., Records, A.R., and Gross, D.C. 2006. Characterization of the regulatory roles of syrF and salA in syringomycin and syringopeptin production by P. syringae pv. syringae. Journal of Bacteriology 188:3290-3298. https://doi.org/10.1128/JB.188.9.3290-3298.2006
Wang, N., Lu, S.-E., Wang, J.L., Chen, J.Z., and Gross, D.C. 2006. The expression of genes encoding lipodepsipeptide phytotoxins by Pseudomonas syringae pv. syringae is coordinated in response to plant signal molecules. Molecular Plant-Microbe Interactions 19:257‑269 https://doi.org/10.1094/MPMI-19-0257
Wang, N., Lu, S.-E., Yang, Q., Sze, S.-H., and Gross, D.C. 2006. Identification of the syr-syp box in the promoter regions of genes dedicated to syringomycin and syringopeptin production by Pseudomonas syringae pv. syringae B301D. Journal of Bacteriology 188:160-168. https://doi.org/10.1128/JB.188.1.160-168.2006
2005
Lu, S.E., Wang, N., Wang, J.L., Chen, J.Z., and Gross, D.C. 2005. Oligonucleotide microarray analysis of the SalA regulon controlling phytotoxin production by Pseudomonas syringae pv. syringae. Molecular Plant-Microbe Interactions 15:43-53. https://doi.org/10.1094/MPMI-18-0324
Academic and Professional Experience
7/2013-present: Associate Professor, Citrus Research and Education Center/Department of Microbiology and Cell Science, University of Florida, Lake Alfred, USA
1/2007-6/2013: Assistant Professor, Citrus Research and Education Center/Department of Microbiology and Cell Science, University of Florida, Lake Alfred, USA
9/2005-12/2006: Postdoctoral Research Associate, Department of Plant and Microbial Biology, University of California, Berkeley, USA
7/1995-8/1998: Research Assistant, Department of Plant Protection, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Shandong, China